Enhance immune function and broad-spectrum antibacterial effect ----- Osthole 98%

The main active components of Fructus Cnidii extract include pinene, camphene, Bornyl isovalerate, isoborneol, methoxyeugenol, cindimine, cnidiadin, isopimpinelline, etc.

Physical Property

The low content of conventional 35% and 50% is yellow green powder, and the high content is white acicular crystalline powder. Soluble in alkali solution, methanol, ethanol, chloroform, acetone, ethyl acetate and boiling petroleum ether, insoluble in water and petroleum ether, MP 83℃~84℃,bp. 145℃~150℃.

Pharmacological Efficacy

Osthol has antispasmodic, hypotensive, antiarrhythmic, enhancing immune function and broad-spectrum antibacterial effects. Warming the kidney, strengthening yang, drying dampness, dispelling wind and killing insects. For impotence, palace cold, infertility, cold and wet belt, wet nose and low back pain; External treatment: eczema of vulva, vaginal itching of women, trichomonal vaginitis.

Pharmacological Action

1. Effect of antiviral microorganism. Fructus Cnidii has inhibitory effect on Trichophyton flocculus, Bacillus gypsum like and Bacillus wool in test tube. It can prolong the life of chicken embryo by 6 hours for Newcastle virus cultured in chicken embryo. Fructus Cnidii extract (1:2) has strong killing effect on Trichomonas vaginalis in vitro. However, it has also been reported that liver extract is used as culture medium, 10% and 20% Fructus Cnidii decoction had no or very weak killing effect on Trichomonas vaginalis

2. Sex hormone like effect. Continuous subcutaneous injection of ethanol extract of Fructus Cnidii for 32 days can prolong the estrous period, shorten the estrous interval, make the ovaries and uterus of ovariectomized mice appear estrous period, and increase the weight of prostate, seminal vesicle and levator ani muscle (mice), Osthol extract has androgen - like effect and no corrosive effect on rabbit vaginal mucosa

3. Insecticidal effect on spermatozoa: prepare 20%, 25%, 30%, 35% and 40% suspensions of Fructus Cnidii extract powder with normal saline, place it at room temperature for 48 hours, 200 revolutions / heart separation for 10 minutes, filter the supernatant and store it at 4 ℃. Observe the spermicidal effect with the drug. It can be seen that the spermicidal effect increases with the increase of the drug concentration, and the minimum effective concentration for inhibiting human spermatozoa within 20 seconds is 30%, Every milliliter of semen is mixed with different doses of Fructus Cnidii extract powder, and the minimum effective dose to inhibit sperm activity is 250 ~ 300mg. The experiment shows that Fructus Cnidii may become a vaginal contraceptive

4. Anti allergy animal experiments show that the acetone extract of Fructus Cnidii has a strong anti histamine effect. It can significantly antagonize the intestinal muscle contraction caused by histamine, chronic reactive substances and 2% egg white. Osthol has a strong inhibitory effect on passive skin allergic reaction in mice, It can inhibit the degranulation of rat peritoneal mast cells induced by trichosanthin

5. Antiarrhythmic effect the aqueous extract of Fructus Cnidii has preventive effects on chloroform induced ventricular fibrillation in mice, calcium chloride induced ventricular fibrillation in rats and aconitine induced arrhythmia in rats, and has obvious therapeutic effect on the latter. This shows that the aqueous extract of Fructus Cnidii has antiarrhythmic effect. It can effectively resist aconitine and promote the opening of sodium channel Accelerate the ectopic rhythm of Na + influx, suggesting that the aqueous extract of Fructus Cnidii can significantly inhibit the influx of Na + and Ca + + in cardiomyocyte membrane, so as to play an anti arrhythmia role

6. The study on the effect of Osthol on the respiratory system shows that Osthol has a strong bronchodilator effect. Osthol can increase the pulmonary perfusion flow of guinea pigs, and its effect is stronger than aminophylline. Total coumarin of Osthol is administered by gavage to guinea pigs, It can prolong the attack time of asthma in guinea pigs caused by histamine phosphate and acetylcholine chloride. In vitro experiments show that Osthol total coumarin has a strong antagonistic effect on histamine induced tracheal contraction in isolated guinea pigs. The above effects can be blocked by propranolol and have no effect on heart rate and blood pressure in anesthetized animals, suggesting that Osthol total coumarin may selectively excite bronchial smooth muscle β 2 receptor, producing antispasmodic and antiasthmatic effects. Intragastric administration of total coumarin from Fructus Cnidii to mice can significantly increase the excretion of phenol red, suggesting that this product has a strong expectorant effect

7. Local anesthetic effect: Cnidium monnieri Decoction and alcohol precipitation (100%) can completely block the generation of action potential of isolated sciatic nerve of toad, and its intensity is the same as that of 10% procaine. It can restore normal conduction function after drug withdrawal; It has the effect of infiltration anesthesia on guinea pigs, which can be enhanced by adrenaline hydrochloride, but the time of local anesthesia is shorter than that of 10% procaine; It has spinal anesthesia effect on rabbits, but has no surface anesthesia effect on rabbit cornea

8. The anti mutagenic effect experiment showed that the water-soluble extract of Fructus Cnidii had strong anti mutagenic effect on cancer inducer aflatoxin B1; It can reduce the frequency of SCE (sister chromosome exchange in isolated cells) induced by positive dingmycin and cyclophosphamide; It has an antagonistic effect on chromosome aberration and micronucleus of mouse bone marrow cells induced by cyclophosphamide, and its aberration rate and micronucleus cell rate gradually decreased with the increase of the dose of Fructus Cnidii extract

9. Other effects: the extract of Fructus Cnidii can drive Ascaris lumbricoides. The sewage of Fructus Cnidii can kill larvae